Understanding Microbial Identification
In the realm of microbiology, the identification of bacteria and fungi is crucial for various research and clinical applications. Our services begin with the identification of bacteria and archaea through advanced techniques such as 16s rRNA sequencing. This method allows for precise categorization of microbial species. Fungi identification follows a similar path, utilizing rRNA internal transcribed spacer (ITS) sequencing for accurate results.
Diving Deeper with Whole Genome Sequencing
Once initial identification is complete, we offer whole genome sequencing and comprehensive analysis. This phase includes gene identification and comparative genomics, employing methods like average nucleotide identity (ANI), average amino acid identity (AAI), digital DNA-DNA hybridization (dDDH), and phylogenomic analysis. These techniques, alongside Venn diagrams, facilitate the identification of both shared and unique genes within microbial communities, enhancing our understanding of their relationships and functions.
Advanced Testing Techniques for Thorough Analysis
We do not stop at genetic identification; our suite of services integrates traditional testing methods including gram stains, biochemical assays, and antibiotic sensitivity tests. Furthermore, we employ medium throughput phenotypic analysis with Biolog Gen III plates and high throughput analysis with Biolog phenotype microarrays. To ensure thorough characterization, we also conduct a variety of chemotaxonomic analyses, such as those for pigments, respiratory quinones, polar lipids, and fatty acid methyl esters.
With over 20 years of dedicated work, our collection boasts well-characterized bacteria and yeasts, meticulously identified by Lycoming College microbiology students. We aim to provide a robust framework for understanding microbial diversity and functionality across various environments.