Isolate gDNA, PCR amplify 16s rRNA gene from gDNA with universal primers 27f & 1492r. Sanger sequence with 27f to confirm correct DNA.

Combine with other samples on a shared Illumina MiSeq run (2 x 300b) at a core sequencing facility. Target is 50-100x coverage.

Assemble genome using SPAdes in PATRIC. Upload assembly to RAST for annotation, confirm 16s rRNA sequence by BLAST. Deposit WGS assembly into GenBank and raw reads into SRA archive at NCBI